Directed evolution of an alcohol dehydrogenase for the desymmetric reduction of 2,2-disubstituted cyclopenta-1,3-diones by enzymatic hydrogen transfer
Hongliu Zhang, Liangyan Zhu, Jinhui Feng, Xiangtao Liu, Xi Chen, Qiaqing Wu, Dunming Zhu
Abstract
Directed evolution of carbonyl reductase TbADH created mutant Tb2 with balanced activity toward ethyl secodione and isopropanol, enabling the desymmetric reduction of ethyl secodione to give (13 R ,17 S )-ethyl secol with 94% yield, >99% ee and >99% de.
Topics & Concepts
Yield (engineering)Alcohol dehydrogenaseChemistryAlcoholCatalysisEnzymeReduction (mathematics)EthanolMutantMedicinal chemistryCombinatorial chemistryStereochemistryOrganic chemistryBiochemistryMaterials scienceMathematicsGeneMetallurgyGeometryEnzyme Catalysis and ImmobilizationAmino Acid Enzymes and MetabolismMetabolism and Genetic Disorders