Human FKBP5 Negatively Regulates Transcription through Inhibition of P-TEFb Complex Formation
Dheerendra Pratap Mall, Subham Basu, Koushik Ghosh, Nidhi Kumari, Abhishake Lahiri, Sandip Paul, Debabrata Biswas
Abstract
biochemical assays, coupled with cell-based assays, showed a strong negative effect of FKBP5 on P-TEFb-mediated phosphorylation of diverse substrates. Consistently, FKBP5 knockdown showed enhanced P-TEFb complex formation that led to increased global RNA polymerase II C-terminal domain (CTD) phosphorylation, expression of proliferation-related genes, and subsequent proliferation. Thus, our results show an important role for FKBP5 in negative regulation of P-TEFb functions within mammalian cells.
Topics & Concepts
P-TEFbBiologyCell biologyRNA polymerase IITranscription (linguistics)Transcription factorFKBP5Glucocorticoid receptorGene expressionMolecular biologyGeneticsGenePromoterLinguisticsPhilosophyPARP inhibition in cancer therapyHeat shock proteins researchSignaling Pathways in Disease