Constant Enzymatic <i>in situ</i> Production of H<sub>2</sub>O<sub>2</sub> for an Unspecific Peroxygenase by an <scp>l–</scp>Amino Acid Oxidase
Simon Last, Tobias Heinks, N.L. Dietz, Simon Koopmeiners, Gabriele Fischer von Mollard, Martin J. Weissenborn, Jan von Langermann
Abstract
Abstract Unspecific Peroxygenases have been the subject of a lot of research in the recent past as they offer opportunities for green hydroxylation reactions using hydrogen peroxide (H 2 O 2 ) as donor. A striking problem of UPOs is their low stability against their own co‐substrate H 2 O 2 . In order to prevent the deactivation, we developed an enzyme cascade with an l ‐amino acid oxidase (LAAO) to produce hydrogen peroxide in situ at a constant low concentration, aiming for much higher lifespan and therefore longer working time of the enzyme. This way, we are able to convert a variety of substrates with conversion rates up to 95%.