Evaluation of strategies for overcoming trifluoroacetic acid ionization suppression resulted in single-column intact level, middle-up, and bottom-up reversed-phase LC-MS analyses of antibody biopharmaceuticals
Maria Khalikova, Laimonas Skarbalius, Denis K. Naplekov, Siddharth Jadeja, František Švec, Juraj Lenčo
Topics & Concepts
Trifluoroacetic acidChemistryFormic acidChromatographyMass Spectrometry Techniques and ApplicationsProtein purification and stabilityAnalytical Chemistry and Chromatography