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DsrA Modulates Central Carbon Metabolism and Redox Balance by Directly Repressing <i>pflB</i> Expression in <i>Salmonella</i> Typhimurium

Rui Dong, Yuan Liang, Shoukui He, Yan Cui, Chunlei Shi, Yiping He, Xianming Shi

2022Microbiology Spectrum13 citationsDOIOpen Access PDF

Abstract

Small RNA DsrA plays an important role in defending against oxidative stress in bacteria. In this study, we identified a novel target ( pflB , encoding pyruvate-formate lyase) of DsrA and demonstrated its potential regulatory mechanism in S. Typhimurium by transcriptome analysis. In silico prediction revealed a direct base pairing between DsrA and pflB mRNA, which was confirmed in site-directed mutagenesis experiments.

Topics & Concepts

BiologyRNase PGene expressionGeneticsGeneBiochemistryCell biologyRNABacterial Genetics and BiotechnologyBacteriophages and microbial interactionsSalmonella and Campylobacter epidemiology
DsrA Modulates Central Carbon Metabolism and Redox Balance by Directly Repressing <i>pflB</i> Expression in <i>Salmonella</i> Typhimurium | Litcius