Single-cell RNA transcriptomic analysis identifies Creb5 and CD11b-DCs as regulator of asthma exacerbations
Xiaojie Liu, Keilah Garcia Netto, Leon A. Sokulsky, Lujia Zhou, Huisha Xu, Chi Liu, Ming Wang, Huaqi Wang, Hui Li, Guojun Zhang, Paul S. Foster, Fuguang Li, Ming Yang
Abstract
DC cells. Analysis of conserved regulon factors further identified Creb5 as a shared regulon between the exacerbation groups. Creb5 siRNAs attenuated HDM, CRE or RSV-induced asthma exacerbation. scRNA-seq multidimensional analysis of immune clusters identified gene pathways that were conserved between the exacerbation groups. We propose that these analyses provide a strong framework that could be used to identify specific therapeutic targets in multifaceted pathologies.
Topics & Concepts
RegulonImmune systemTranscriptomeImmunologyExacerbationBiologyAsthmaHouse dust miteGene silencingSmall interfering RNARNAGeneAllergenRegulation of gene expressionGeneticsAllergyGene expressionIL-33, ST2, and ILC PathwaysSingle-cell and spatial transcriptomicsAsthma and respiratory diseases