In vivo engineering of murine T cells using the evolved adeno-associated virus variant Ark313
William A. Nyberg, Charlotte H. Wang, Jonathan Ark, Chang Liu, Sylvanie Clouden, Anita Qualls, Sofia Caryotakis, E. Wells, Katherine Simon, Celeste Garza, P Bernard, Maya Lopez-Ichikawa, Zhongmei Li, Jin Seo, Gabriella R. Kimmerly, Joseph J. Muldoon, Peixin Amy Chen, Mingcheng Li, Hong-Erh Liang, Kelly Kersten, Alan Rosales, Nicholas F. Kuhn, Chun Ye, James M. Gardner, Ari B. Molofsky, Roberto R. Ricardo-González, Aravind Asokan, Justin Eyquem
Abstract
T cells, including naive T cells. Ark313-mediated transgene delivery presents an efficient approach to target mouse T cells in vivo and a resource for the interrogation of T cell biology and for immunotherapy applications.
Topics & Concepts
BiologyTransgeneIn vivoAdeno-associated virusCre recombinaseCD8Gene deliveryImmunotherapyMolecular biologyReporter geneVirologyCell biologyGenetic enhancementGenetically modified mouseImmune systemGeneImmunologyVector (molecular biology)Recombinant DNAGeneticsGene expressionVirus-based gene therapy researchCAR-T cell therapy researchRNA Interference and Gene Delivery