A small-sized benzothiazole–indolium fluorescent probe: the study of interaction specificity targeting c-MYC promoter G-quadruplex structures and live cell imaging
Bo‐Xin Zheng, Meng-Ting She, Wei Long, Yong-Yu Xu, Yi-Han Zhang, Xuan‐He Huang, Wenjie Liu, Jin-Qiang Hou, Wing‐Leung Wong, Yu‐Jing Lu
Abstract
A small-sized c-MYC promoter G-quadruplex selective fluorescent BZT-Indolium binding ligand was demonstrated for the first time as a highly target-specific and photostable probe for in vitro staining and live cell imaging and it was found to be able to inhibit the amplification of the c-MYC G-rich sequence (G-quadruplex) and down-regulate oncogene c-MYC expression in human cancer cells (HeLa).
Topics & Concepts
BenzothiazoleFluorescenceG-quadruplexChemistryLive cell imagingDNACellBiophysicsMolecular biologyBiologyBiochemistryPhysicsQuantum mechanicsDNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery