An Evaluation Study for Chlorophyll Estimation Techniques

Abstract

A field experiment was conducted to evaluate the efficacy of two destructive and two nondestructive\nchlorophyll estimation techniques for two vegetable crops (aroma tomato Solanum lycopersicum L. and green\npepper Capsicum annuum L.) in three different environments (open field, lath house and plastic house) to\nindicate the best method of chlorophyll estimation. After 80 days from sowing chlorophyll estimation was\nimplemented first by nondestructive methods using SPAD (SPAD-502 Plus Konica Minolta Tokyo, Japan)\nand atLEAF chlorophyll meter (at-LEAF FT-GREEN-LLC Willington, DE, USA) instruments, then\nthe same leaves were cut and chlorophyll estimation were done by destructive methods using two organic\nsolvents ( 80% acetone and 100% ethanol). Results demonstrate significant differences between SPAD and\natLEAF data for tomato and pepper plants where higher levels with atLEAF instrument comparing to\nSPAD chlorophyll meter. Higher chlorophyll levels were recorded in open field plants comparing to lath and\nplastic houses plants. Chlorophyll a content was related to carotenoid levels in both destruction methods.\nThere was positive correlation between SPAD and atLEAF. Nondestructive methods could be used as an alternative method for destructive methods.

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