Single-nucleotide resolution of <i>N</i><sup>6</sup>-adenine methylation sites in DNA and RNA by nitrite sequencing
Yasaman Mahdavi‐Amiri, Kimberley Chung Kim Chung, Ryan Hili
Abstract
Chemoselective deamination of adenine in the presence of N 6 -methyladenine using nitrite enables single-nucleotide resolution sequencing of N 6 -adenine methylation sites in DNA and RNA.
Topics & Concepts
DeaminationDNAGuanineRNACpG siteChemistryNucleic acidHypoxanthineDNA methylationNucleotideMethylationBiochemistryMolecular biologyBiologyEnzymeGeneGene expressionRNA modifications and cancerEpigenetics and DNA MethylationCancer-related gene regulation