A 3D Spheroid Model for Glioblastoma
Joris Guyon, Lætitia Andrique, Nadège Pujol, Gro Vatne Røsland, Gaëlle Recher, Andréas Bikfalvi, Thomas Daubon
Abstract
Two-dimensional (2D) cell cultures do not mimic in vivo tumor growth satisfactorily. Therefore, three-dimensional (3D) culture spheroid models were developed. These models may be particularly important in the field of neuro-oncology. Indeed, brain tumors have the tendency to invade the healthy brain environment. We describe herein an ideal 3D glioblastoma spheroid-based assay that we developed to study tumor invasion. We provide all technical details and analytical tools to successfully perform this assay.
Topics & Concepts
SpheroidGlioblastomaBrain tumorIn vivoNeuroscienceBiologyCell culturePathologyComputer scienceComputational biologyCancer researchMedicineGenetics3D Printing in Biomedical ResearchCell Image Analysis TechniquesCancer Cells and Metastasis