Site-selective and inducible acylation of thrombin using aptamer-catalyst conjugates
Jordi F. Keijzer, Judith J. Firet, Bauke Albada
Abstract
Two acyl-transfer catalysts were conjugated to thrombin-binding DNA aptamers to acylate thrombin. Modification occurred site-selectively on Lys (≫Ser) residues proximal to the respective aptamer-thrombin interface, was selective for thrombin in the presence of other proteins, and the activity of both DNA-catalysts could be controlled by an external trigger.
Topics & Concepts
AptamerThrombinAcylationConjugateCatalysisChemistryConjugated systemCombinatorial chemistryDNABiophysicsBiochemistryMolecular biologyOrganic chemistryBiologyPlateletImmunologyMathematical analysisMathematicsPolymerAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA Interference and Gene Delivery