Optimization of T-DNA configuration with UBIQUITIN10 promoters and tRNA–sgRNA complexes promotes highly efficient genome editing in allotetraploid tobacco
Manoj Kumar, Dana Ayzenshtat, Adar Marko, Samuel Bocobza
Topics & Concepts
BiologyTransgeneGenome editingGeneticsGeneCas9Nicotiana tabacumSubgenomic mRNATransformation (genetics)MutantPromoterCRISPRGene knockoutComputational biologyGene expressionCRISPR and Genetic EngineeringChromosomal and Genetic VariationsRNA and protein synthesis mechanisms