Effect of Extraction Method on Antimicrobial Activity Against Staphylococcus Aureus of Tapak Liman (Elephantopus Scaber L.) Leaves
Ike Widyaningrum, Nugroho Wibisono, Anggun Hari Kusumawati
Abstract
This study aims to compare the antibacterial activity of Staphylococcus aureus from tapak liman leaf extract using different extraction methods, maceration, and soxhletation. Maceration is a type of cold extraction, without using temperature. Meanwhile, soxhletation is a type of extraction that involves temperature in the process. The solvent used in this research is ethanol 96%. It is known that 96% of ethanol has a good safety level. The results of the phytochemical screening of tapak liman leaf extract from the two methods showed no difference in phytochemical content. The phytochemical content of tapak liman leaf extract from the two extraction methods are alkaloids, flavonoids, phenolics, and tannins. To determine the antibacterial activity in this study, the ZOI method using discs was used. The tool used to determine the inhibition ability of tapak liman leaf extract was a ruler with an accuracy level of 0.5 mm. Based on the results of the antibacterial activity test, it is known that the tapak liman leaf extract using the sokhletation method provides better inhibitory ability than the tapak liman leaf extract using the maceration method.