scGR-seq: Integrated analysis of glycan and RNA in single cells
Haruki Odaka, Haruka Ozaki, Hiroaki Tateno
Abstract
Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems. For complete details on the use and execution of this profile, please refer to Minoshima et al. (2021).
Topics & Concepts
TranscriptomeGlycanComputational biologyBiologyRNA-SeqProfiling (computer programming)Multicellular organismRNAInduced pluripotent stem cellProgenitor cellSingle-cell analysisCellCell biologyGeneStem cellMolecular biologyGeneticsGlycoproteinComputer scienceGene expressionEmbryonic stem cellOperating systemSingle-cell and spatial transcriptomicsGlycosylation and Glycoproteins ResearchRNA Research and Splicing