NBD-based fluorescent probes for separate detection of cysteine and biothiols <i>via</i> different reactivities
Haojie Huang, Xiuru Ji, Yaqing Jiang, Changyu Zhang, Xueying Kang, Jiqin Zhu, Lu Sun, Long Yi
Abstract
NBD-based fluorescent probes that can separately detect cysteine and biothiols via different reactivities have been rationally designed and synthesized. The probes can be applied to kinetically distinguish cysteine from other biothiols at 30 min, and to detect all biothiols at 3 h in living cells.
Topics & Concepts
FluorescenceCysteineChemistryCombinatorial chemistryBiochemistryBiophysicsStereochemistryBiologyPhysicsEnzymeQuantum mechanicsSulfur Compounds in BiologyFolate and B Vitamins ResearchPregnancy and Medication Impact