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Computational Reverse Engineering Analysis for Scattering Experiments (CREASE) on Vesicles Assembled from Amphiphilic Macromolecular Solutions

Ziyu Ye, Zijie Wu, Arthi Jayaraman

2021JACS Au17 citationsDOIOpen Access PDF

Abstract

generated from vesicles with dispersity in all relevant dimensions, resembling real experiments. After successful validation of CREASE, we compare the CREASE-determined dimensions against those obtained from the traditional approach of fitting the scattering intensity profile to relevant analytical model in SASVIEW package. We show that CREASE performs better than or as well as the core-multishell analytical model's fitting in SASVIEW in determining vesicle dimensions with dispersity. We also show that CREASE provides structural information beyond those possible from traditional scattering analysis using the core-multishell model, such as the distribution of solvophilic monomers between the vesicle wall's inner and outer layers in the vesicle wall and the chain-level packing within each vesicle layer.

Topics & Concepts

VesicleScatteringAmphiphileSmall-angle X-ray scatteringDispersityPolymerMacromoleculeBiological systemDynamic light scatteringIn silicoMolecular dynamicsChemistryMaterials scienceChemical physicsCopolymerNanotechnologyOpticsPhysicsPolymer chemistryComputational chemistryNanoparticleComposite materialBiologyMembraneBiochemistryGeneAdvanced Polymer Synthesis and CharacterizationProtein purification and stabilityRNA Interference and Gene Delivery
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