Antioxidant and antimicrobial properties of squalene from Symphytum officinale and chlorogenic acid from trifolium pratense

Abstract

For the prevention of the development of a number of CVDs the systematic use of dietary supplements containing antioxidant substances is promising. Squalene and chlorogenic acid are considered among the candidate substances. The natural sources of which are plants, for squalene is comfrey and for chlorogenic acid is meadow clover. The purpose of this work was to evaluate the presence of antimicrobial and antioxidant properties of squalene and chlorogenic acid obtained from in vitro root culture extracts of the respective plants. The implementation of this goal was carried out on the basis of the Laboratory for Biotesting of Natural Nutraceuticals ( Kemerovo State University, Russia). Conventional assessment methods were used: antimicrobial activity - using the disk diffusion method; antioxidant activity - by trapping ABTS radical cations. As a result, it was found that the BAS solutions under consideration did not show the ability to stimulate the viability of pathogenic, conditionally pathogenic strains. Squalene emulsion (800 –1600 µmol/L) showed high activity against E. coli B-8208. A solution of chlorogenic acid (100–1600 µmol/L) showed moderate activity against St. aureus B-6646, and from 400 to 1600 µmol/l - to P. putida B-1827. The results of the analysis showed that chlorogenic acid showed high antioxidant activity in comparison with ascorbic acid, and the squalene emulsion did not show antioxidant properties, i.e. did not catch ABTS radical cations. Presumably, the antioxidant activity of squalene is not expressed in the capture of free radicals, but in the ability to act on biomolecules that lead to oxidative stress. Chlorogenic acid is a promising antioxidant substance that does not stimulate the development of pathogenic strains, therefore, it is important to use it in the manufacture of prophylactic dietary supplements.

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