Antibacterial Efficacy of Sodium Hypochlorite, Ozonated Water, and 980 nm Diode Laser Used for Disinfection of Root Canal against Enterococcus faecalis: A Microbiological Study
Jagriti Kushwah, Rahul Mishra, Vishal Bhadauria
Abstract
Aim: Evaluation and comparison of the antibacterial efficacy of sodium hypochlorite, ozonated water, diode laser, and diode laser in combination with ozonated water against Enterococcus faecalis. Materials and methods: One hundred and twenty extracted premolar teeth were sectioned at the cementoenamel junction and root canals were prepared using step-back technique with K-file up to #40. The teeth were arbitrarily allocated to four groups and stowed into brain heart infusion broth containing a microbial suspension of E. faecalis. Group I samples were irrigated with 3% sodium hypochlorite. Group II samples were irrigated with ozonated water. Group III samples were irrigated firstly by distilled water followed by laser irradiation. Group IV samples were irrigated by ozonated water followed by laser irradiation. After the treatment, the teeth were kept in vials containing 2 mL of nutrient broth. The vials were then incubated at 37C for 24 h. Standard methods were then used to identify grown colonies. Statistical analysis: Kruskal-Wallis test, Mann-Whitney test, Chi-square test, and Wilcoxon signed-rank test were used to measure the colonyforming units (CFUs) obtained at the end of the incubation period among the various groups for the evaluation of antibacterial efficacy of various disinfection protocols.