Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides
Mengdie Ren, Yiru Hou, Danli Peng, Hui Li, Xuxiang Zhang, Lei Qiao, Xiaoshuang Wang, Yongmei Jiang, Faming Wu, Wang Gang
Abstract
This study investigates two extraction methods-ultrasound-assisted extraction (UAE) and compound enzyme extraction (CE)-for the isolation of Lonicera macranthoides polysaccharides (LMP) and evaluates their physicochemical properties, bioactivity, and therapeutic potential. The optimization of extraction conditions was performed using single-factor experiments and response surface methodology. The yield of LMP from UAE was optimized to 8.02 % ± 0.12 %, while the yield from CE was 10.03 % ± 0.32 %. Both polysaccharide extracts were characterized in terms of chemical composition, monosaccharide composition, molecular weight, thermal stability, and functional group analysis. Notably, UAE-LMP exhibited superior antioxidant, hypolipidemic, nitrite scavenging, and anti-tumor activities compared to CE-LMP. The ultrasound-assisted method enhanced the stability and bioactivity of LMP, with improved water- and oil-holding capacities and the integrity of the active groups. Our findings demonstrate that the extraction method significantly influences the structural and functional properties of LMP, suggesting that UAE may be a more effective approach for extracting bioactive polysaccharides with potential applications in medicine and nutrition.