1,4-Naphthoquinone Is a Potent Inhibitor of IRAK1 Kinases and the Production of Inflammatory Cytokines in THP-1 Differentiated Macrophages
Ismail Sami Mahmoud, Ma’mon M. Hatmal, Duaa Abuarqoub, Ezaldeen Esawi, Hiba Zalloum, Suha Wehaibi, Hamdi Nsairat, Walhan Alshaer
Abstract
using structural-based docking and dynamic simulation analysis. Also, 1,4-naphthoquinone was found to effectively inhibit the growth of cancer cell lines overexpressing IRAK1. Furthermore, 1,4-naphthoquinone potently suppressed the production and secretion of key proinflammatory cytokine proteins IL-8, IL-1β, IL-10, TNF-α, and IL-6 in LPS-stimulated PMA-induced human THP-1 macrophages. In conclusion, 1,4-naphthoquinone is an effective inhibitor of IRAK1 kinases and their mediated inflammatory cytokines production in LPS-stimulated PMA-induced human THP-1 macrophages.
Topics & Concepts
Proinflammatory cytokineNaphthoquinonePlumbaginKinaseChemistryTHP1 cell lineIn vitroStaurosporineEmodinIn silico1,4-NaphthoquinoneBiochemistryPharmacologyCell biologyCell cultureProtein kinase AInflammationBiologyImmunologyGeneticsGeneOrganic chemistryBioactive Compounds and Antitumor AgentsFree Radicals and AntioxidantsATP Synthase and ATPases Research