A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues
Daniel Spitzer, Maryam I. Khel, Tim Pütz, Jenny Zinke, Xiaoxiong Jia, Kathleen Sommer, Katharina Filipski, Frits Thorsen, Thomas M. Freiman, Stefan Günther, Karl H. Plate, Patrick N. Harter, Stefan Liebner, Yvonne Reiss, Mariangela Di Tacchio, Sylvaine Guérit, Kavi Devraj
Topics & Concepts
Flow cytometryCell sortingImmunolabelingCell typeCell biologyMicrogliaBiologyCellCell culturePericyteImmunologyEndothelial stem cellInflammationIn vitroImmunohistochemistryGeneticsBiochemistryBarrier Structure and Function StudiesSingle-cell and spatial transcriptomicsNeuroinflammation and Neurodegeneration Mechanisms