Genetic encoding of ε-<i>N</i>-<scp>l</scp>-lactyllysine for detecting delactylase activity in living cells
Yanan Sun, Yanchi Chen, Yaxin Xu, Yu‐Qing Zhang, Minghao Lu, Manjia Li, Liyan Zhou, Tao Peng
Abstract
-L-lactylated recombinant proteins and the construction of fluorescent and luminescent probes for detecting delactylases in living cells. Using these probes, we demonstrate sirtuin 1 as a potential delactylase for non-histone proteins.
Topics & Concepts
Encoding (memory)ChemistryComputational biologyBiologyNeuroscienceProtein Hydrolysis and Bioactive PeptidesSupramolecular Self-Assembly in MaterialsBiochemical effects in animals