A super-resolution and transmission electron microscopy correlative approach to study intracellular trafficking of nanoparticles
Teodora Andrian, Yolanda Muela, Lídia Delgado, Lorenzo Albertazzi, Sílvia Pujals
Abstract
endosomal escape). The proposed protocol can be applied to fluorescently labelled NPs and/or cargo, including those not detectable by TEM alone. Our studies are of great relevance to obtain important information on NP trafficking, and crucial for the design of more complex nanomaterials aimed at cytoplasmic/nucleic drug delivery.
Topics & Concepts
EndosomeCytoplasmNanotechnologyIntracellularContext (archaeology)BiophysicsCellular compartmentNanoparticleMicroscopyNanomaterialsFluorescence microscopeEthylene glycolDrug deliveryTransmission electron microscopyMaterials scienceChemistryCell biologyBiologyFluorescenceCellBiochemistryPhysicsOrganic chemistryPaleontologyQuantum mechanicsOpticsAdvanced Electron Microscopy Techniques and ApplicationsAdvanced Fluorescence Microscopy TechniquesNear-Field Optical Microscopy