Substrate access path-guided engineering of <scp>l</scp>-threonine aldolase for improving diastereoselectivity
Wenlong Zheng, Zhongji Pu, Lanxin Xiao, Gang Xu, Lirong Yang, Haoran Yu, Jianping Wu
Abstract
The path hypothesis cooperates with the CAST/ISM strategy to explore the relationship between the physico-chemical properties of the substrate access path and diastereoselectivity of l -threonine aldolase.
Topics & Concepts
Aldolase ADiastereomerSteric effectsChemistrySubstrate (aquarium)ThreonineEnzymeStereochemistryCombinatorial chemistryBiochemistryBiologySerineEcologyAmino Acid Enzymes and MetabolismEnzyme Structure and FunctionEnzyme Catalysis and Immobilization