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Acinetobacter baumannii Kills Fungi via a Type VI DNase Effector

Jingjing Luo, Xiao Chu, Jing Jie, Yu Sun, Qingtian Guan, Dan Li, Zhao‐Qing Luo, Lei Song

2023mBio45 citationsDOIOpen Access PDF

Abstract

Acinetobacter baumannii is an increasing important nosocomial pathogen that is difficult to combat due to its ability to survive in harsh environments and the emergence of isolates that are resistant to multiple antibiotics. A better understanding of the mechanism underlying the toughness of A. baumannii may identify its Achilles' heel, which will facilitate the development of novel preventive and treatment measures. In this study, our findings show that A. baumannii kills fungi with the DNase effector TafE injected into competitor cells by its type VI secretion system. A. baumannii is protected from the activity of TafE by the immunity protein TaeI, which inactivates the effector by direct binding. Our results suggest that inactivation of its T6SS or effectors may reduce the fitness of A. baumannii and increase the effectiveness of treatment by means such as antibiotics. Furthermore, our finding suggests that targeted degradation of TaeI may be an effective strategy to kill A. baumannii.

Topics & Concepts

Acinetobacter baumanniiEffectorBiologyMicrobiologyType VI secretion systemPathogenBacteriaVirulenceCell biologyGeneGeneticsPseudomonas aeruginosaVibrio bacteria research studiesAntibiotic Resistance in BacteriaEscherichia coli research studies