The Mycobacterium tuberculosis sRNA F6 Modifies Expression of Essential Chaperonins, GroEL2 and GroES
Joanna Houghton, Angela Rodgers, Graham Rose, Alexandre D’Halluin, Terry Kipkorir, Declan Barker, Simon J. Waddell, Kristine B. Arnvig
Abstract
Control of gene expression via small regulatory RNAs (sRNAs) is poorly understood in one of the most successful pathogens, Mycobacterium tuberculosis. Here, we present an in-depth characterization of the sRNA F6, including its expression in different infection models and the differential gene expression observed upon deletion of the sRNA. Our results demonstrate that deletion of F6 leads to dysregulation of the two essential chaperonins GroEL2 and GroES and, moreover, indicate a role for F6 in the long-term survival and persistence of M. tuberculosis in the human host.
Topics & Concepts
Mycobacterium tuberculosisBiologyChaperoninGene expressionGeneTuberculosisGroESGeneticsMicrobiologyGroELMedicinePathologyEscherichia coliRNA and protein synthesis mechanismsRNA modifications and cancerTuberculosis Research and Epidemiology