Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF
Takahiro Furuta, Kenta Yamauchi, Shinichiro Okamoto, Megumu Takahashi, Soichiro Kakuta, Yoko Ishida, Aya Takenaka, Atsushi Yoshida, Yasuo Uchiyama, Masato Koike, Kaoru Isa, Tadashi Isa, Hiroyuki Hioki
Abstract
eSF. Our multi-scale neuronal imaging incorporates (1) brain-wide macroscopic observation, (2) mesoscopic circuit mapping, (3) microscopic subcellular imaging, and (4) EM imaging of nanoscopic structures, allowing seamless integration of structural information from the brain to synapses. We applied this technique to three neural circuits of two different species, mouse striatofugal, mouse callosal, and marmoset corticostriatal projection systems, and succeeded in simultaneous interrogation of their circuit structure and synaptic connectivity in a targeted way. Our multi-scale neuronal imaging will significantly advance the understanding of brain-wide connectivity by expanding the scales of objects.