Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China
Yulan Huang, Xiaobo Ma, Shihan Zeng, Liang Fu, Heping Xu, Xiaoyan Li
Abstract
New Delhi metallo-β-lactamase-13 (NDM-13) is an NDM variant that was first identified in 2015 and has not been detected in Salmonella species prior to this study. Here we describe the first identification of a Salmonella Rissen strain SR33 carrying bla NDM-13 . The aim of this study was to molecularly characterize SR33’s antimicrobial resistance and virulence features as well as investigate the genetic environment of bla NDM-13 . The Salmonella Rissen SR33 strain was isolated from a patient with fever and diarrhea. SR33 belonged to ST469, and it was found to be multidrug-resistant (MDR) and to carry many virulence genes. Phylogenetic analysis showed that SR33 shared a close relationship with most of the Chinese S . Rissen ST469 strains. bla NDM-13 was located in a transmissible IncI1 plasmid pNDM13-SR33. Sequence analysis of bla NDM-13 -positive genomes downloaded from GenBank revealed that a genetic context (ΔIS Aba125 - bla NDM-13 - ble MBL -trpF) and a hybrid promoter (consisting of −35 sequences provided by IS Aba125 and −10 sequences) were conserved. IS Aba125 was truncated by IS 1294 in three plasmids carrying bla NDM-13 , including pNDM13-SR33. To our knowledge, this is the first report of bla NDM-13 carried by Salmonella . The emergence of bla NDM-13 in a clinical MDR S . Rissen ST469 strain highlights the critical need for monitoring and controlling the dissemination of bla NDM-13 . bla NDM-13 carried by a transmissible IncI1 plasmid may result in an increased risk of bla NDM-13 transmission. IS 1294 may be involved in the movement of bla NDM-13 .