Enhancing oleic acid content in two commercially released peanut varieties through marker‐assisted backcross breeding
Mangesh P. Jadhav, Malagouda D. Patil, Mahesh R Hampannavar, S Venkatesh, Pavana Dattatreya, Kenta Shirasawa, Pasupuleti Janila, Manish K. Pandey, Rajeev K. Varshney, Ramesh Bhat
Abstract
Abstract Two elite cultivars of peanut ( Arachis hypogaea L.), GPBD 4 and G 2–52, with high productivity, oil content, resistance to late leaf spot [ Phaeoisariopsis personata (Berk. & Curt) V. Arx.] (LLS) and rust ( Puccinia arachidis Speg.) diseases were improved for oleic acid content using marker‐assisted backcrossing. Since both the recurrent parents already possessed the mutant allele at AhFAD2A , only mutant allele at AhFAD2B was transferred from the donor ‘SunOleic 95R’ (oleate of 80.6%). Three rounds of backcrossing with foreground selection using allele‐specific polymerase chain reaction (PCR) and Kompetitive allele‐specific PCR (KASP) assay identified a large number of plants homozygous for the mutant allele at AhFAD2B in BC n F 2 generations. Evaluation of the advanced generations could identify six and 10 lines with significantly higher oleate than GPBD 4 and G 2–52, respectively. Considering the yield, shelling percentage, oil, and oleate content, the most promising lines HOBC 2 GPS_7 and HOBC 2 G2S_5 were selected with 112 and 142% oleate recovery over GPBD 4 and G 2–52, respectively. Double digest restriction‐site‐associated DNA sequencing of these superior lines showed background genome recovery of 77.5 and 69.0%, respectively. These advanced breeding lines with high oleate (∼80%), resistance to LLS and rust and high productivity are under further trials for possible release as varieties for commercial cultivation.