Antibacterial activity and cytocompatibility evaluation of the antimicrobial peptide Nal-P-113-loaded graphene oxide coating on titanium
Qian Cheng, Ran Lu, Xin Wang, Su Chen
Abstract
The initialization of the gingival crevicular barrier epithelium facing the exposed implant surface is reported to take 1-2 weeks, and peri-implant inflammation mediated by bacterial infection has been an increasing concern. In this study, we developed a novel composite coating containing graphene oxide (GO) and the antimicrobial peptide (AMP) Nal-P-113 on smooth titanium surface, and then investigated the antibacterial properties and cytocompatibility of the coating. The results showed that Nal-P-113 exhibited a slow and continuous drug release in vitro from the composite coating. The Nal-P-113-loaded GO coating demonstrated excellent antibacterial properties against both Gram-positive Streptococcus mutans (S. mutans) and Gram-negative Porphyromonas gingivalis (P. gingivalis), without no obvious cytotoxicity to human gingival fibroblast (HGF) cells. The Nal-P-113-loaded GO coating need further optimization for anti-infective and wound healing promoting purpose of peri-implant tissues.