Transcriptomic analysis of the innate immune response to in vitro transfection of plasmid DNA
Eric Warga, Jared L. Anderson, Matthew S. Tucker, Emily Harris, Jacob Elmer
Abstract
). Indeed, overexpression of one CSG (IFI16) significantly decreased transfection efficiency in HEK-293T cells.
Topics & Concepts
TransfectionHEK 293 cellsLipofectamineJurkat cellsBiologyMolecular biologyDownregulation and upregulationGene deliveryInnate immune systemCell cultureCell biologyImmune systemT cellGeneImmunologyGeneticsVector (molecular biology)Recombinant DNARNA Interference and Gene DeliveryVirus-based gene therapy researchCAR-T cell therapy research