Propagation of goose primordial germ cells in vitro relies on FGF and BMP signalling pathways
Dadakhalandar Doddamani, Bence Lázár, Kennosuke Ichikawa, Tuanjun Hu, Lorna Taylor, Elen Gócza, Eszter Patakiné Várkonyi, Mike J. McGrew
Abstract
Mitotically active embryonic reproductive cells, the primordial germ cells (PGCs), are an ideal cell type for cryopreserving functional reproductive cells for avian species. Their low number in the avian embryo, however, renders cryopreservation and germline transmission methodologies difficult. Here, we develop a culture medium for the long-term in vitro culture of PGCs from the goose, Anser anser domesticus. In contrast to chicken, goose PGC self-renewal is dependent on the TGF-β family member, BMP4, and, conversely, is inhibited by Activin A. An RNA transcriptome analysis reveals commonalities between cultured PGCs from chicken and goose species, including a marked transcriptional difference between male and female goose PGCs. In vitro propagated goose PGCs are amenable to genetic modification using DNA transposons and colonising the gonads of xenogeneic sterile host embryos. These data demonstrate that the conservation and cryopreservation of the genetic diversity of the >1400 endangered bird species using PGCs remains a valid possibility.