Selective cell encapsulation, lysis, pico-injection and size-controlled droplet generation using traveling surface acoustic waves in a microfluidic device
Kirk Mutafopulos, Peter J. Lu, Ryan Garry, Pascal Spink, David A. Weitz
Abstract
We generate droplets in a microfluidic device using a traveling surface acoustic wave (TSAW), and control droplet size by adjusting TSAW power and duration. We combine droplet production and fluorescence detection to selectively-encapsulate cells and beads; with this active method, the overwhelming majority of single particles or cells are encapsulated individually into droplets, contrasting the Poisson distribution of encapsulation number that governs traditional, passive microfluidic encapsulation. In addition, we lyse cells before selective encapsulation, and pico-inject new materials into existing droplets.
Topics & Concepts
MicrofluidicsLysisMaterials scienceEncapsulation (networking)NanotechnologyCell encapsulationTraveling waveOptoelectronicsChemistryComputer sciencePolymer chemistryBiochemistryMathematicsSelf-healing hydrogelsComputer networkMathematical analysisInnovative Microfluidic and Catalytic Techniques InnovationMicrofluidic and Capillary Electrophoresis ApplicationsMicrofluidic and Bio-sensing Technologies