Simultaneous detection of 5-methylcytosine and 5-hydroxymethylcytosine at specific genomic loci by engineered deaminase-assisted sequencing
Neng‐Bin Xie, Min Wang, Tong‐Tong Ji, Xia Guo, Fang‐Yin Gang, Ying Hao, Li Zeng, Yafen Wang, Yu‐Qi Feng, Bi‐Feng Yuan
Abstract
values, we can deduce the precise levels of C, 5mC, and 5hmC at particular genomic locations. We successfully used EDA-seq to simultaneously measure C, 5mC, and 5hmC at specific loci within human lung cancer tissue and their normal counterpart. The results from EDA-seq demonstrated a strong concordance with those obtained from the combined application of BS-seq and ACE-seq methods. EDA-seq eliminates the need for bisulfite treatment, DNA oxidation or glycosylation and uniquely enables simultaneous quantification of C, 5mC and 5hmC at the same genomic locations.
Topics & Concepts
5-Hydroxymethylcytosine5-MethylcytosineComputational biologyDNAgenomic DNADNA sequencingSequencing by ligationBiologyGeneticsGenomic libraryDNA methylationBase sequenceGeneGene expressionEpigenetics and DNA MethylationRNA modifications and cancerCancer-related molecular mechanisms research