Tuning the Properties of Natural Promiscuous Enzymes by Engineering Their Nano-environment
Carolina I. Giunta, Isabel Cea‐Rama, Sandra Alonso, Manon L. Briand, Rafael Bargiela, Cristina Coscolín, Philippe F.-X. Corvini, Manuel Ferrer, J. Sanz‐Aparicio, Patrick Shahgaldian
Abstract
, acetonitrile). The versatility of the method was assessed with two additional substrate-promiscuous and structurally different enzymes, for which improvements in enantioselectivity and stability were confirmed. We expect this method to promote the use of supramolecularly engineered promiscuous enzymes in industrially relevant biocatalytic processes.
Topics & Concepts
Substrate (aquarium)BiocatalysisProtein engineeringEnzymeContext (archaeology)Supramolecular chemistryEnantioselective synthesisCombinatorial chemistryChemistryCatalytic efficiencyMaterials scienceNanotechnologyCatalysisOrganic chemistryBiologyMoleculeIonic liquidPaleontologyEcologyEnzyme Catalysis and ImmobilizationBiochemical and Structural CharacterizationCarbohydrate Chemistry and Synthesis