β-Glucanase Family Genes Promote Resistance to Sheath Blight in Rice by Inhibiting the Permeability of Plasmodesmata
Tiange Zhou, Ying He, Xiao Han, Qian Sun, Yuanhu Xuan
Abstract
Rice sheath blight (ShB) caused by Rhizoctonia solani is one of the most serious diseases that threatens rice ( Oryza sativa ) production. However, the mechanisms of defense against ShB in rice remain largely unknown. In this study, we identified that the expression levels of β- glucanase ( OsBGL ) family genes sensitively respond to infection by R. solani, and OsBGL s positively regulate rice resistance to ShB. In addition, OsBGL2 colocalized with AtPDCB1 at the plasmodesmata (PD) and limited the PD permeability. The level of callose accumulation in osbgls mutants and overexpressors was examined, and OsBGL s were found contribute to callose accumulation. Taken together, these data suggest that OsBGL s can regulate the deposition of callose at the PD to reduce its permeability to defend itself against ShB. Through the identification of these genes and the elucidation of their functions, this research fills the gap in the mechanism of PD permeability in rice ShB resistance.