Characterization of Pseudomonas aeruginosa <scp>l,d</scp> -Transpeptidases and Evaluation of Their Role in Peptidoglycan Adaptation to Biofilm Growth
Inès Hugonneau-Beaufet, Jean‐Philippe Barnier, Stanislas Thiriet-Rupert, Sylvie Létoffé, Jean‐Luc Mainardi, Jean‐Marc Ghigo, Christophe Beloin, Michel Arthur
Abstract
Active-site cysteine LDTs form a functionally heterologous family of enzymes that contribute to the biogenesis of the bacterial cell envelope through formation of peptidoglycan cross-links and through the dynamic anchoring of lipoproteins to peptidoglycan. Here, we report the role of three P. aeruginosa LDTs that had not been previously characterized. We show that these enzymes contribute to resistance to the bactericidal activity of EDTA and to the adaptation of cell envelope polymers to conditions that prevail in biofilms. These results indicate that LDTs should be considered putative targets in the development of drug-EDTA associations for the control of biofilm-related infections.