In-frame editing of transcription factor gene RDD1 to suppress miR166 recognition influences nutrient uptake, photosynthesis, and grain quality in rice
Masao Iwamoto
Abstract
Abstract The transcription factor-encoding gene RDD1 increases the uptake of nutrient ions, photosynthetic activity under ambient and high CO 2 conditions, and grain productivity, and microRNA166 (miR166) regulates its transcript levels. This study found that CRISPR/Cas9 genome editing of rice plants to inhibit miR166– RDD1 transcript pairing ( R1 -Cas plants) increased RDD1 transcript levels, NH 4 + and PO 4 3− uptake, and photosynthetic activity under high CO 2 conditions in rice. However, the panicle weight of the R1 -Cas plants decreased compared with the wild-type (WT) plants. Adversely, changes in environmental conditions, such as high CO 2 or high temperatures, showed insignificant differences in the panicle weight between the WT and R1 -Cas plants despite a largely increased panicle weight observed in the transgenic RDD1 -overexpressing plants. Moreover, both the R1 -Cas and transgenic RDD1 -overexpressing plants that were matured in a growth chamber demonstrated an improved grain appearance quality or a decrease in the number of chalky grains compared with the WT plants. These results suggest that the in-frame mutagenesis of RDD1 to suppress miR166– RDD1 transcript pairing contributes to the improved grain appearance of rice.