Enhanced biomineralization and protein adsorption capacity of 3D chitosan/hydroxyapatite biomimetic scaffolds applied for bone-tissue engineering
Nguyen Kim Nga, Lai Thi Thanh Tam, Nguyễn Thu Hà, Pham Hung Viet, Trần Quang Huy
Abstract
. Results showed that the 3D CS/HAp scaffolds exhibited highly porous structures with an average pore size of 265 μm, and mean porosity of 75.01%, respectively; the tensile strength of the scaffolds was 2.45 MPa, matching well with that of cancellous bone. The addition of HAp into the CS matrix efficiently decreased the swelling percentage of the CS/HAp scaffolds and retained the suitable degradation rate of the composite scaffolds; the degradation percentage of the CS/HAp scaffolds was 46.37% after 28 days immersed in a physiological solution. The CS/HAp scaffolds demonstrated a higher biomineralization capability than that of the CS scaffolds, releasing a bone-like apatite layer on their surface after 15 days of incubation in simulated body fluids. The presence of HAp mimicking biological apatite in the composite scaffolds facilitated a higher protein adsorption capability, compared to that of the CS scaffolds. The obtained results suggest that the CS/HAp scaffolds have great potential as biocompatible materials for BTE applications.