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Label-Free Multiphoton Imaging of Microbes in Root, Mineral, and Soil Matrices with Time-Gated Coherent Raman and Fluorescence Lifetime Imaging

Jang Hyuk Lee, Rachel Hestrin, Erin Nuccio, Keith D. Morrison, Christina Ramon, Ty Samo, Jennifer Pett‐Ridge, Sonny Ly, Ted A. Laurence, Peter Weber

2022Environmental Science & Technology29 citationsDOIOpen Access PDF

Abstract

in a soil microcosm. Our results show that this multimodal approach holds significant promise for rhizosphere and soil science research.

Topics & Concepts

Brachypodium distachyonFluorescence-lifetime imaging microscopyFluorescenceFluorophoreRaman spectroscopyRhizosphereChemistryAutofluorescenceMaterials scienceOpticsBacteriaPhysicsBiologyBiochemistryGeneticsGeneGenomeSpectroscopy Techniques in Biomedical and Chemical ResearchAdvanced Fluorescence Microscopy TechniquesBee Products Chemical Analysis