Fundamental insights in early‐stage inclusion body formation
Julian Kopp, Benjamin Bayer, Christoph Slouka, Gerald Striedner, Mark Dürkop, Oliver Spadiut
Abstract
Early-stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod-shaped protein aggregates, a potential sponge-like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod-shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations.
Topics & Concepts
Inclusion (mineral)Inclusion bodiesStage (stratigraphy)Morphology (biology)Escherichia coliFunction (biology)ChemistryBiologyBiophysicsCell biologyBiochemistryZoologyMineralogyPaleontologyGeneBacterial Genetics and BiotechnologyProtein purification and stabilityBacteriophages and microbial interactions