Litcius/Paper detail

Cytotoxicity and Bioactivity of Calcium Silicate-based Cements in a Culture of Stem Cells from the Apical Papilla.

Adriana Knorr, Letícia Boldrin Mestieri, Lucas Siqueira Pinheiro, Roberta Almeida Mendes, Pedro Antônio González Hernández, Fernando Branco Barletta, Fabiana Soares Grecca

2021PubMed14 citationsDOIOpen Access PDF

Abstract

Introduction: study evaluated the cytotoxicity and bioactivity of commonly-used calcium silicate-based cements in a culture of stem cells from the apical papilla (SCAPs). Materials and Methods: ), where cells were exposed to 1:4 diluted extract on days 1 and 7. Minimum Essential Eagle's Medium alpha modification was used as control. ANOVA and Tukey's post hoc test were used to compare the different cements at each experimental time point. Results: <0.05). Conclusion: All evaluated calcium silicate-based cements demonstrated cell viability and bioactivity, suggesting that these (bio)materials may be indicated for use in regenerative dentine-pulp complex procedures.

Topics & Concepts

Viability assayAlkaline phosphataseCalcium silicateCytotoxicityMTT assayChemistryNuclear chemistryCalcium hydroxideMineral trioxide aggregateAndrologyMolecular biologyCalciumIn vitroDentistryMaterials scienceBiochemistryBiologyMedicineEnzymeOrganic chemistryPhysical chemistryComposite materialEndodontics and Root Canal TreatmentsDental Implant Techniques and OutcomesBone Tissue Engineering Materials