Chemo-enzymatic production of base-modified ATP analogues for polyadenylation of RNA
Rachel M. Mitton-Fry, Jannik Eschenbach, Helena Schepers, René Rasche, Mehmet Erguven, Daniel Kümmel, Andrea Rentmeister, Nicolas V. Cornelissen
Abstract
-, or C8-modified ATP analogues. These can be incorporated into RNA using template independent poly(A) polymerase. C2-halogenated ATP analogues were incorporated best, with incorporations of 300 to >1000 nucleotides forming hypermodified poly(A) tails.
Topics & Concepts
PolyadenylationBase (topology)ChemistryEnzymeRNAProduction (economics)Post-transcriptional modificationBiochemistryRNA editingGeneMathematicsEconomicsMathematical analysisMacroeconomicsRNA and protein synthesis mechanismsRNA modifications and cancerRNA Research and Splicing