Open-source microscope add-on for structured illumination microscopy
Mélanie T. M. Hannebelle, Esther Raeth, Samuel M. Leitão, Tomáš Lukeš, Jakub Pospíšil, Chiara Toniolo, Olivier F. Venzin, Antonius Chrisnandy, Prabhu Prasad Swain, Nathan Ronceray, Matthias P. Lütolf, Andrew C. Oates, Guy M. Hagen, Theo Lasser, Aleksandra Rađenović, John D. McKinney, Georg E. Fantner
Abstract
Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to upgrade existing optical microscopes to Structured Illumination super-resolution Microscopes (SIM). The openSIM is an open-hardware system, designed and documented to be easily duplicated by other laboratories, making super-resolution modality accessible to facilitate innovative research. The add-on approach gives a performance improvement for pre-existing lab equipment without the need to build a completely new system.