Sugar based cationic magnetic core–shell silica nanoparticles for nucleic acid extraction
Tammar Hussein Ali, Amar Mousa Mandal, Thorsten Heidelberg, Rusnah Syahila Duali Hussen
Abstract
) for easy isolation by an external magnetic field. The particles enabled a high DNA particle loading ratio of 30-45 wt% (MNP/DNA ratio), reflecting an efficient extraction process. A high desorption rate (7 min) with more than 86% of unchanged DNA loading was recorded, indicating low damage to the target extract.
Topics & Concepts
Nucleic acidMagnetic nanoparticlesTriethylene glycolNanoparticleChemistrySurface modificationPyrophosphateExtraction (chemistry)Chemical engineeringMaterials scienceNanotechnologyOrganic chemistryBiochemistryEngineeringPhysical chemistryEnzymeAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionElectrochemical sensors and biosensors