Nematodes Degrade Extracellular Antibiotic Resistance Genes by Secreting DNase II Encoded by the <i>nuc-1</i> Gene
Wenhua Dong, Yi Liu, Jie Hou, Jianying Zhang, Jiang Xu, Kun Yang, Lizhong Zhu, Daohui Lin
Abstract
This study investigated the degradation performance and mechanism of extracellular antibiotic resistance genes (eARGs) by nematodes using batch degradation experiments, mutant strain validation, and phylogenetic tree construction. Caenorhabditis elegans, a representative nematode, effectively degraded approximately 99.999% of eARGs ( tetM and kan ) in 84 h and completely deactivated them within a few hours. Deoxyribonuclease (DNase) II encoded by nuc-1 in the excretory and secretory products of nematodes was the primary mechanism. A neighbor-joining phylogenetic tree indicated the widespread presence of homologs of the NUC-1 protein in other nematodes, such as Caenorhabditis remanei and Caenorhabditis brenneri, whose capabilities of degrading eARGs were then experimentally confirmed. C. elegans remained effective in degrading eARGs under the effects of natural organic matter (5, 10, and 20 mg/L, 5.26–6.22 log degradation), cation (2.0 mM Mg 2+ and 2.5 mM Ca 2+, 5.02–5.04 log degradation), temperature conditions (1, 20, and 30 °C, 1.21–5.26 log degradation), and in surface water and wastewater samples (4.78 and 3.23 log degradation, respectively). These findings highlight the pervasive but neglected role of nematodes in the natural decay of eARGs and provide novel approaches for antimicrobial resistance mitigation biotechnology by introducing nematodes to wastewater, sludge, and biosolids.