Engineered aldolases catalyzing stereoselective aldol reactions between aryl-substituted ketones and aldehydes
Eugenia Chukwu Cornelius, Michael H. Bartl, Louise Persson, Ruisheng Xiong, Daniela Cederfelt, Farshid Mashayekhy Rad, Thomas Norberg, Sarah Engel, Erik G. Marklund, Doreen Dobritzsch, Mikael Widersten
Abstract
An A129G/R134V/S166G triple mutant of fructose 6-phosphate aldolase (FSA) from Escherichia coli was further engineered with the goal to generate new enzyme variants capable of catalyzing aldol reactions between aryl substituted ketones and aldehydes.
Topics & Concepts
Aldol reactionAldolase AArylStereoselectivityChemistryEscherichia coliMutantCatalysisFructoseEnzymeCombinatorial chemistryOrganic chemistryStereochemistryBiochemistryGeneAlkylCarbohydrate Chemistry and SynthesisEnzyme Catalysis and ImmobilizationEnzyme Structure and Function