ATP stabilised and sensitised calcium phosphate nanoparticles as effective adjuvants for a DNA vaccine against cancer
Bing Sun, Xiaohui Zhao, Wenxi Gu, Wenxi Gu, Pei Cao, Fatemeh Movahedi, Yanheng Wu, Zhi Ping Xu, Wenyi Gu, Wenyi Gu
Abstract
stabiliser for CP and immunological adjuvant, and applied the ATP-modified CP (ACP) NPs to the DNA vaccine. ACP NP-enhanced cellular uptake and improved transfection efficiency of DNA vaccine, and further showed the ability to activate DCs that are critical for them to prime T cells in cancer immunotherapy. As a result, a higher level of antigen-specific antibody with stronger tumour growth inhibition was achieved in mice immunised with the ACP-DNA vaccine. Overall, this one-step synthesised ACP NPs are an efficient nano-delivery system and nano-adjuvant for cancer DNA vaccines.
Topics & Concepts
DNA vaccinationAdjuvantImmunogenicityCancer immunotherapyCancer vaccineDNATransfectionCancer cellAntigenImmune systemCancer researchChemistryCancerImmunotherapyBiologyBiochemistryImmunologyPlasmidGeneticsGeneImmunotherapy and Immune ResponsesRNA Interference and Gene DeliveryNanoplatforms for cancer theranostics