Biological and Rheological Properties of Collagen Cross-Linked with Glutaraldehyde
Yu. A. Nashchekina, O. A. Lukonina, Diana M. Darvish, А. В. Нащекин, V. Yu. Elokhovskii, V. E. Yudin, Natalia Mikhailova
Abstract
Abstract We study the effect of the concentration of glutaraldehyde that can cross-link type I collagen molecules on the collagen stability, mechanical strength, and cytotoxicity. It is shown that collagen containing 10 and 18% of glutaraldehyde exhibits the highest stability in physiological conditions. The results of rheological analysis demonstrate that, for the minimal concentration of glutaraldehyde (1%), mechanical characteristics of collagen hydrogels are improved significantly. However, the glutaraldehyde concentration higher than 10% reduces the vitality of cells by 50%.
Topics & Concepts
GlutaraldehydeRheologyMaterials scienceSelf-healing hydrogelsMechanical strengthCytotoxicityChemical engineeringComposite materialChemistryPolymer chemistryChromatographyIn vitroBiochemistryEngineeringCollagen: Extraction and CharacterizationEngineering Technology and Methodologiesbiodegradable polymer synthesis and properties